King's College London
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Figure 4 and D4: Gradual Varnish Removal

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posted on 2023-03-22, 13:47 authored by Jakub NedbalJakub Nedbal

This dataset is the source for Figures 4 and D4 in the manuscript "Visualising Varnish Removal for Conservation of Paintings by Fluorescence Lifetime Imaging (FLIM)". It contains measurements of painting surface acquired after incremental cleaning steps using swab application of solvent with or without mechanical action. The dataset contains the raw measurement of the painting surface before, during, and after varnish removal tests. The raw data was processed by the MATLAB processFLIM.m script in the collection called "SPAD Linearization Code". The processed data was fit in TRI2, and produced into graphical outputs using the MATLAB makeFLIMglob.m script included with this dataset. stichImages.m and destitchImages.m scripts are to combine several measurements together for FLIM analysis in TRI2 and to separate the results, respectively. The fit results and the graphical outputs used in the publication are included in the dataset.

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

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EP/R511559/1

History

Data collection from date

Feb 17, 2022

Data collection to date

Feb 17, 2022

Collection method

Macroscope, QuantiCAM, 440 nm pulsed picosecond laser @ 25 MHz, 475 nm long-pass emission filter

Copyright owner

Jakub Nedbal

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    Faculty of Natural, Mathematical & Engineering Sciences

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