King's College London
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Figure 5: Painting Sample Cross-Sections

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posted on 2023-03-22, 13:47 authored by Jakub NedbalJakub Nedbal

This dataset was used in the preparation of Figure 5 in the manuscript "Visualising Varnish Removal for Conservation of Paintings by Fluorescence Lifetime Imaging (FLIM)". The dataset contains the raw measurements and processed outputs of measurements of painting sample cross-sections. These cross-sections have been mounted in resin and imaged using a 4X 0.1 NA air objective on a FLIM microscope equipped with a SPAD camera. The data set contains the raw measurement on the cross-sections The raw data was processed by the MATLAB processFLIM.m script in the collection called "SPAD Linearization Code". The processed data was fit in TRI2, and produced into graphical outputs using the MATLAB makeFLIMglob.m script included with this dataset. stichImages.m and destitchImages.m scripts are to combine several measurements together for FLIM analysis in TRI2 and to separate the results, respectively. The fit results and the graphical outputs used in the publication are included in the dataset.

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

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EP/R511559/1

History

Data collection from date

Feb 15, 2022

Data collection to date

Feb 15, 2022

Collection method

FLIM microscope with 4x 0.1 NA air objective and a 550/88 emission filter. Laser excitation light source had a 445 nm center wavelength. Image acquisition was performed with the QuantiCAM SPAD camera.

Copyright owner

Jakub Nedbal

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    Faculty of Natural, Mathematical & Engineering Sciences

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