Figure 5: Raw data, MATLAB scripts, and processed microalgae FLIM image stills

Image still data in transmission and fluorescence lifetime contrast have been acquired with microalgae

Samples consisted of C. vulgaris and D. quadricauda cells, either treated with DCMU or left untreated. The cells were live and dark acclimed and part treated with DCMU herbicide. The fluorescence lifetime of those cells is increased due DCMU blocking electron acceptor oxidation and thus reaction center quenching.

The images were created using the accompanying makeFLIM.m script for MATLAB. The calibration data from the SPADlinarization package (https://doi.org/10.18742/21325584) will be needed to run this script.

This dataset is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"