King's College London
Browse
algae_stills.tgz (115.72 MB)

Figure 5: Raw data, MATLAB scripts, and processed microalgae FLIM image stills

Download (115.72 MB)
dataset
posted on 2024-03-06, 16:47 authored by Jakub NedbalJakub Nedbal

Image still data in transmission and fluorescence lifetime contrast have been acquired with microalgae

Samples consisted of C. vulgaris and D. quadricauda cells, either treated with DCMU or left untreated. The cells were live and dark acclimed and part treated with DCMU herbicide. The fluorescence lifetime of those cells is increased due DCMU blocking electron acceptor oxidation and thus reaction center quenching.

The images were created using the accompanying makeFLIM.m script for MATLAB. The calibration data from the SPADlinarization package (https://doi.org/10.18742/21325584) will be needed to run this script.

This dataset is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

Find out more...

EP/X525571/1

History

Data collection from date

11. Mar 2021

Data collection to date

11. Mar 2021

Collection method

Fluorescence lifetime microscopy with QuantiCam SPAD array sensor. 60x 1.49 NA oil objective.

Copyright owner

Jakub Nedbal

Usage metrics

    Faculty of Natural, Mathematical & Engineering Sciences

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC