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Figure 6, C3, D5: Natural Resin Varnish Test Samples

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posted on 2023-03-22, 13:47 authored by Jakub NedbalJakub Nedbal

This dataset is the source for Figures 6, C3, and D5 in the manuscript "Visualising Varnish Removal for Conservation of Paintings by Fluorescence Lifetime Imaging (FLIM)". It contains measurements of test samples coated with Dammar and Mastic varnishes. Samples have undergone ambient or accelerated aging. The data set contains the raw measurement of the test sample surfaces. The raw data was processed by the MATLAB processFLIM.m script in the collection called "SPAD Linearization Code". The processed data was fit in TRI2, and produced into graphical outputs using the MATLAB makeFLIMglob.m script included with this dataset. stichImages.m and destitchImages.m scripts combine several measurements together for FLIM analysis in TRI2 and then separate the results, respectively. The fit results and the graphical outputs used in the publication are included in the dataset. The phasor plot data in Fig. C3 was created by the included script makePhasor.m.

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

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EP/R511559/1

History

Data collection from date

Feb 21, 2022

Data collection to date

Feb 21, 2022

Collection method

Macroscope, QuantiCAM, 440 nm pulsed picosecond laser @ 25 MHz, 475 nm long-pass emission filter

Copyright owner

Jakub Nedbal

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    Faculty of Natural, Mathematical & Engineering Sciences

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