Figure 6: Raw data, MATLAB scripts, and processed time series video of diffusing C. vulgaris cells with FLIM contrast

Image time series data with fluorescence lifetime contrast have been acquired with microalgae C. vulgaris. A mixture of cells treated with DCMU and untreated control cells was used The fluorescence lifetime of the DCMU-treated cells was increased due DCMU blocking electron acceptor oxidation and thus reaction center quenching.

The continuous stream of photon timestamps (11-Mar-2021_19_36_17_1000us_00001.mat) was segmented into individual 1-second integration time frames using the accompanying divideStream.m script for MATLAB.

The video of the time series frames, analyzed in TRI2, was created by another script createVideo.m. The calibration data from the SPADlinarization package (https://doi.org/10.18742/21325584) will be needed to run this script.

This dataset is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"