King's College London
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Cvulgaris_timeseries.tgz (501.27 MB)

Figure 6: Raw data, MATLAB scripts, and processed time series video of diffusing C. vulgaris cells with FLIM contrast

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posted on 2024-03-06, 16:47 authored by Jakub NedbalJakub Nedbal

Image time series data with fluorescence lifetime contrast have been acquired with microalgae C. vulgaris. A mixture of cells treated with DCMU and untreated control cells was used The fluorescence lifetime of the DCMU-treated cells was increased due DCMU blocking electron acceptor oxidation and thus reaction center quenching.

The continuous stream of photon timestamps (11-Mar-2021_19_36_17_1000us_00001.mat) was segmented into individual 1-second integration time frames using the accompanying divideStream.m script for MATLAB.

The video of the time series frames, analyzed in TRI2, was created by another script createVideo.m. The calibration data from the SPADlinarization package (https://doi.org/10.18742/21325584) will be needed to run this script.

This dataset is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

Find out more...

EP/X525571/1

History

Data collection from date

11. Mar 2021

Data collection to date

11. Mar 2021

Collection method

Fluorescence lifetime microscopy with QuantiCam SPAD array sensor. 60x 1.49 NA oil objective.

Copyright owner

Jakub Nedbal

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    Faculty of Natural, Mathematical & Engineering Sciences

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