King's College London
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Figure 7: Raw data, MATLAB scripts, and processed videos of lightsheet image stacks of algae cells

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posted on 2024-03-06, 16:47 authored by Jakub NedbalJakub Nedbal

3D volume stacks of images of algae cells embedded in agarose. C. vulgaris and D. quadricauda cells were either treated with DCMU, left untreated as controls and a mixture of treated and untreated C. vulgaris cells. The fluorescence lifetime of the DCMU-treated cells was increased due DCMU blocking electron acceptor oxidation and thus reaction center quenching. There are three folders in the package:

  • Dquadricauda_lightsheet
  • Cvulgaris_lightsheet
  • Cvulgaris_mixture_lightsheet

Each folder contains a MATLAB script makeFLIM.m, which produces videos and graphs from the SPAD output data analyzed in TRI2. The calibration data from the SPADlinearization package (https://doi.org/10.18742/21325584) will be needed to run this script.

This dataset is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

Find out more...

EP/X525571/1

History

Data collection from date

2. Jun 2021

Data collection to date

23. Jun 2021

Collection method

Fluorescence lifetime microscopy with QuantiCam SPAD array sensor using a 60x 1.49 NA oil objective. Illumination with the Mizar Tilt Lightsheet module.

Copyright owner

Jakub Nedbal

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    Faculty of Natural, Mathematical & Engineering Sciences

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