<p>This is the dataset used for calibrating the SPAD camera timing skew and to generate an instrument response function for the microscope. This dataset is an input to the MATLAB <em>SPADlinearization.m</em> script. It was used in the preparation of the data in manuscript "Visualising Varnish Removal for Conservation of Paintings by Fluorescence Lifetime Imaging (FLIM)".</p>
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<p>The measurement was obtained with a quenched fluorescein sample on a TCSPC microscope.</p>
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<p>The dataset is used to create calibration structures for the microscopic optical system. The calibration structures can be reproduced by running the <em>SPADlinearization.m</em> script on the raw data. The resulting calibration structure and the scripts are available in another dataset in this collection called "SPAD Linearization Code". </p>
Funding
EP/R511559/1
Development of wide-field TCSPC fluorescence microscopy for cell membrane studies
Biotechnology and Biological Sciences Research Council