Instrument Response Function Measurement for Macroscopic Painting Imaging
This is the dataset used for calibrating the SPAD camera timing skew and to generate an instrument response function for the FLIM macroscopic imaging system. This dataset is an input to the MATLAB SPADlinearization.m script and is associated with the data in the article "Visualising Varnish Removal for Conservation of Paintings by Fluorescence Lifetime Imaging (FLIM)".
The measurement was obtained from the macroscopic FLIM setup used without an emission filters and with a piece of office paper as the scattering sample. The dataset is used to create calibration structures for the two optical systems (macroscopic and microscopic). The calibration structures can be reproduced by running the SPADlinearization.m script. Both the resulting calibration structure and the scripts are available in another dataset in this collection called "SPAD Linearization Code".
Funding
Development of wide-field TCSPC fluorescence microscopy for cell membrane studies
Biotechnology and Biological Sciences Research Council
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