Raw data associated with the article Piper, C. and Green, J.B.A. "Cap-to-bell stage molar tooth morphogenesis occurs through proliferation-independent sulcus sharpening and condensation-associated tension in the dental papilla", Journal of Anatomy 2025

This archive contains the raw data associated with the paper "Cap-to-bell stage molar tooth morphogenesis occurs through proliferation-independent sulcus sharpening and condensation-associated tension in the dental papilla". It shows, using mouse molar explant inhibition and cut-and-recoil experiments, that cap-to-bell stage morphogenesis is largely proliferation-independent (sulcus sharpening entirely so) (Fig.1) and that tension in the mesenchyme of the dental papilla, immediately sub-adjacent to the cusps, rather than compression by the mesenchyme surrounding the whole structure, is what holds the structure in shape (Figs. 2 and 3). Fine mapping of the degree of condensation (Fig.4) shows that it is highest in the mesenchyme of the dental papilla and becomes progressively more focused to the cusp regions, consistent with a key role in cusp shaping. Together these findings overturn the prevailing models of molar morphogenesis, including both cusp and sulcus formation. Files in this archive consist of raw (.tif) versions of images in the figures, similar images that were used to generate the graphs in the figures, morphometric (.morphoj) files containing all the measurements and process used to generate some of the graphs in Fig.1, R-code used to generate the cell packing/density heatmaps, and the StarDist machine-learning model used to segment the images of nucleus (DAPI) stained molar tooth slices to generate the nuclear location data (point cloud) used as input to the R-code.