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Reprogramming astroglia into neurons with hallmarks of fast-spiking parvalbumin-positive interneurons by phospho-site deficient Ascl1

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posted on 2024-09-24, 15:16 authored by Nicolas Marichal NegrinNicolas Marichal Negrin, Sophie Péron, Ana Beltran ArranzAna Beltran Arranz, Chiara Galante, Franciele Franco Scarante, Rebecca Wiffen, Carol Schuurmans, Marisa Karow, Sergio Gascón, Benedikt Berninger

Confocal microscopy images, electrophysiological traces and analysis done in MS Excel. All zipped data folders include readme files explaining how to navigate them.

Funding

Lineage reprogramming of glia into subtype-specific cortical neurons

Wellcome Trust

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In vivo Imaging Genesis and Circuit Integration of Interneurons Engineered from Glia

European Research Council

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German Research Foundation BE 4182/11-1 project number 357058359

CRC1080, project No. 221828878

ERA-NET Neuron grant Brain4Sight, 01EW2202

Rheinland- Pfalz at the Johannes Gutenberg University Mainz (ReALity)

the Inneruniversitäre Forschungsförderung Stufe I of the Johannes Gutenberg University Mainz

Core funding to the Francis Crick Institute from Cancer Research United Kingdom

University attachments

Cancer Research UK

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HFSP Long-Term Fellowship, LT000646/2015

São Paulo Research Foundation (FAPESP) process No. 2021/13515-5

History

Temporal coverage

2018-2024

Data collection from date

2018

Data collection to date

2024

Collection method

Neuroscience techniques in ex vivo mouse brain tissue, including immunohistochemistry, RNAscope, confocal microscopy, and whole-cell patch-clamp electrophysiology.

Language

English

Copyright owner

Benedikt Berninger, King's College London

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    Institute of Psychiatry, Psychology & Neuroscience

    Categories

    Keywords

    ASCL1 overexpressionAscl1SA6Asctocyteslineage reprogrammingfast-spiking interneuronparvalbumin interneuron

    Licence

    CC BY

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