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Cvulgaris_mixture_timeSeries.mov (972.59 kB)

Figure 6A: Diffusing C. vulgaris cells, partly treated with DCMU, and imaged with fluorescence lifetime contrast.

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posted on 2024-03-06, 16:38 authored by Jakub NedbalJakub Nedbal

Video of fluorescence lifetime images acquired at one frame per second and showing diffusing C. vulgaris cells. The cells are live and dark acclimed and part treated with DCMU herbicide. The fluorescence lifetime of those cells is increased due DCMU blocking electron acceptor oxidation and thus reaction center quenching.

This video is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

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EP/X525571/1

History

Data collection from date

11. March 2021

Data collection to date

11. March 2021

Collection method

Fluorescence lifetime microscopy with QuantiCam SPAD array sensor. 60x 1.49 NA oil objective.

Copyright owner

Jakub Nedbal

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    Faculty of Natural, Mathematical & Engineering Sciences

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