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Cvulgaris_ctrl_stack.mp4 (151.28 kB)

Figure 7A: Immobilized C. vulgaris cells, untreated, and imaged with fluorescence lifetime contrast and selective plane illumination. 3D axial stack fly-through.

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posted on 2024-03-06, 16:42 authored by Jakub NedbalJakub Nedbal

Video of fluorescence lifetime images acquired with 1 um axial spacing and showing immobilized live control C. vulgaris cells without DCMU treatment in a 3D fly-through. The cells were live and dark acclimed during the imaging. The fluorescence lifetime of those cells is intrinsic without any drug effect.

This video is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

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EP/X525571/1

History

Data collection from date

21. June 2021

Data collection to date

21. June 2021

Collection method

Fluorescence lifetime microscopy with QuantiCam SPAD array sensor. 60x 1.49 NA oil objective, and Mizar Tilt selective plane illumination module. Cells were immobilized in low melting temperature agarose.

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Jakub Nedbal

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    Faculty of Natural, Mathematical & Engineering Sciences

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