Figure 7C: Mixture of Immobilized C. vulgaris cells either treated with DCMU or not, and imaged with fluorescence lifetime contrast and selective plane illumination. 3D axial stack fly-through.

Video of fluorescence lifetime images acquired with 1 um axial spacing and showing immobilized live C. vulgaris cells in a 3D axial stack fly-through. The cells were live and dark acclimed during the imaging. Part of the cells were untreated and part were treated with DCMU to form a mixture of both cell types. The fluorescence lifetime is differs between the cells in the field of view, depending on their exposure to DCMU.

This video is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"