Figure 7C: Mixture of Immobilized <i>C. vulgaris</i> cells either treated with DCMU or not, and imaged with fluorescence lifetime contrast and selective plane illumination. 3D projection rotating around the X axis.
<p dir="ltr">Video of fluorescence lifetime images acquired with 1 um axial spacing and showing immobilized live <i>C. vulgaris</i> cells in a 3D projection rotating around the X axis. The cells were live and dark acclimed during the imaging. Part of the cells were untreated and part were treated with DCMU to form a mixture of both cell types. The fluorescence lifetime is differs between the cells in the field of view, depending on their exposure to DCMU.</p><p dir="ltr">This video is a supplement to the article "<a href="https://doi.org/10.1038/s41598-024-56122-1" rel="noreferrer" target="_blank">A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos</a>"</p>
Funding
Development of wide-field TCSPC fluorescence microscopy for cell membrane studies
Biotechnology and Biological Sciences Research Council
Fluorescence lifetime microscopy with QuantiCam SPAD array sensor. 60x 1.49 NA oil objective, and Mizar Tilt selective plane illumination module. Cells were immobilized in low melting temperature agarose.