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Cvulgaris_mix_Xprojection.mp4 (712.16 kB)

Figure 7C: Mixture of Immobilized C. vulgaris cells either treated with DCMU or not, and imaged with fluorescence lifetime contrast and selective plane illumination. 3D projection rotating around the X axis.

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posted on 2024-03-06, 16:42 authored by Jakub NedbalJakub Nedbal

Video of fluorescence lifetime images acquired with 1 um axial spacing and showing immobilized live C. vulgaris cells in a 3D projection rotating around the X axis. The cells were live and dark acclimed during the imaging. Part of the cells were untreated and part were treated with DCMU to form a mixture of both cell types. The fluorescence lifetime is differs between the cells in the field of view, depending on their exposure to DCMU.

This video is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

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EP/X525571/1

History

Data collection from date

23. June 2021

Data collection to date

23. June 2021

Collection method

Fluorescence lifetime microscopy with QuantiCam SPAD array sensor. 60x 1.49 NA oil objective, and Mizar Tilt selective plane illumination module. Cells were immobilized in low melting temperature agarose.

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Jakub Nedbal

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    Faculty of Natural, Mathematical & Engineering Sciences

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