Figure 7D: Immobilized <i>D. quadricauda</i> cells, untreated, and imaged with fluorescence lifetime contrast and selective plane illumination. 3D axial stack fly-through.

<p dir="ltr">Video of fluorescence lifetime images acquired with 1 um axial spacing and showing immobilized live <i>D. quadricauda</i> cells in 3D axial fly-through. The cells were live and dark acclimed during the imaging. The fluorescence lifetime of those cells is intrinsic without any drug effect.</p><p dir="ltr">This video is a supplement to the article "<a href="https://doi.org/10.1038/s41598-024-56122-1" rel="noreferrer" target="_blank">A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos</a>"</p>