Figure 8A: Immobilized DPPC GUVs imaged with fluorescence lifetime contrast and selective plane illumination. 3D projection rotating around the Y axis.
Video of fluorescence lifetime images acquired with 1 um axial spacing and showing immobilized live GUVs made of DPPC/cholesterol mixture and labelled with di-4-ANEPPDHQ dye in a 3D projection rotating around the Y axis.
Fluorescence lifetime microscopy with QuantiCam SPAD array sensor. 60x 1.49 NA oil objective, and Mizar Tilt selective plane illumination module. GUVs were immobilized in low melting temperature agarose.