Figure 8B: Immobilized DOPC GUVs imaged with fluorescence lifetime contrast and selective plane illumination. 3D axial stack fly-through.

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posted on 2024-03-06, 16:45 authored by Jakub NedbalJakub Nedbal

Video of fluorescence lifetime images acquired with 1 um axial spacing and showing immobilized live GUVs made of DOPC and labelled with di-4-ANEPPDHQ dye in a 3D stack fly-through.

This video is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"

Funding

Development of wide-field TCSPC fluorescence microscopy for cell membrane studies

Biotechnology and Biological Sciences Research Council

Find out more...

EP/X525571/1

History

Data collection from date

25. May 2021

Data collection to date

25. May 2021

Collection method

Fluorescence lifetime microscopy with QuantiCam SPAD array sensor. 60x 1.49 NA oil objective, and Mizar Tilt selective plane illumination module. GUVs were immobilized in low melting temperature agarose.

Copyright owner

Jakub Nedbal

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Keywords

GUV giant unilamellar vesicle fluorescence lifetime imaging microscopy FLIM single photon avalanche diode SPAD selective plane illumination microscopy SPIM lightsheet microscopy DOPC di-4-ANEPPDHQ

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CC BY

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