SPAD Linearization Code

This software package contains code for Mathworks MATLAB with some code written in C, but compiled for MATLAB for Linux and Windows. The code is used to process data from a wide-field SPAD array sensor with embedded time-to-digital converters. It has two functions: (1) Creating correction datasets from calibration measurements and (2) using these calibration measurements to correct data before their fluorescence lifetime analysis. The dataset contains two calibration sets for a particular SPAD sensor chip. One dataset is for macroscopic imaging, the other one for microscopic imaging. They can be used to analyze the existing data, but calibration measurements for a different chip would need to be made for any data acquired on a different sensor.

More information about the algorithm and its use is available from: Nedbal, J., Della Rocca, F.M., Walker, R., Henderson, R.K., Suhling, K.: Correction of time-resolved SPAD array measurements for accurate single-photon time-resolved biological imaging. In: Advanced Photon Counting Techniques XV, vol. 11721, pp. 65–78 (2021). SPIE.

The code and the data are current as of the publication date of "Visualising Varnish Removal for Conservation of Paintings by Fluorescence Lifetime Imaging (FLIM)". Newer versions of the code may be found at: https://github.com/jnedbal/SPADcorrection